Research Article Open Access

cDNA Cloning and Characterization of the House Dust Mite Allergen Sui p 2

Joshua Evans M. Bajao1 and John Donnie A. Ramos1
  • 1 University of Santo Tomas, Philippines

Abstract

Suidasia pontifica (Sp) is a House Dust Mite (HDM) species found in tropical urban residential areas which causes sensitization of up to 75% of allergic patients. Cloning of allergens from Sp is a significant initial step in understanding the clinical significance of this HDM species. The study describes the cloning and in silico characterization of the group 2 allergen gene from Sp (Sui p 2) with significant homology to Myeloid Differentiation 2 (MD-2) lipid-binding (ML domain) protein allergens. Sui p 2 gene was amplified by Reverse Transcription – PCR using gene-specific primers designed from known HDM group 2 allergens. The amplicons were characterized using NCBI BLAST, ExPASy, MEGA7 and Chimera. Sui p 2 cDNA gene exhibits percent identity to Tyrophagus putrescentiae (65.84%), Aleuroglyphus ovatus (65.69%) and Blomia tropicalis (62.98%). Sui p 2 gene contains 405 bp open reading frame with untranslated regions on its 5’ and 3’ end. Furthermore, structure-based percent identity of Sui p 2 to the group 2 allergen of D. farinae and D. pteronyssinus is 47.62% and 46.83%, respectively. Predicted protein structure reveals a putative immunoglobulin fold consisting of preserved cysteine residues, hydrophobic core and two antiparallel β-sheets containing several TLR4, LPS and IgE binding sites. The results suggest that Sui p 2 may play an important role in HDM induced allergy and may be involved in the induction of the innate immune system. Characterization of Sui p 2 is important to evaluate properly its use for allergy component resolved diagnosis and allergen-specific immunotherapy.

American Journal of Biochemistry and Biotechnology
Volume 16 No. 2, 2020, 222-234

DOI: https://doi.org/10.3844/ajbbsp.2020.222.234

Submitted On: 30 October 2019 Published On: 14 May 2020

How to Cite: Bajao, J. E. M. & Ramos, J. D. A. (2020). cDNA Cloning and Characterization of the House Dust Mite Allergen Sui p 2. American Journal of Biochemistry and Biotechnology, 16(2), 222-234. https://doi.org/10.3844/ajbbsp.2020.222.234

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Keywords

  • Allergen
  • House Dust Mite
  • Innate Immunity
  • T-cell Epitope
  • Toll-Like Receptors