Research Article Open Access

Inhibitory Effect of Crude Walnut Leaf Polysaccharides on Oxidative Damage of Proteins and Proliferation of SGC-7901 Cells

Shuqing Yang1, Zhike Xie1, Shuyan Yu1, Ming He1, Qing Liu1, Haifang Xiao1 and Yuanda Song1
  • 1 Shandong University of Technology, China

Abstract

In this paper, we studied the inhibitory effect of crude Walnut Leaf Polysaccharides (WLP) on free radical-induced oxidative damage of proteins and anti-proliferation activity on human Gastric Cancer cells (SGC-7901). The oxidative degradation of Bovine Serum Albumin (BSA), mouse brain proteins (MBP) and Mouse Liver Proteins (MLP) induced by hydroxyl radical and alcoxyl radical was analyzed by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE). In addition, MTT method was used to analyze the effect of WLP on cell viabilities of SGC-7901 cells. The results indicated that WLP significantly inhibited the oxidative damage of BSA induced by hydroxyl radical within the concentration range of 0.5~20 mg/mL. Similar protective effects were found in hydroxyl radical-induced oxidative damage on mouse tissue proteins. WLP inhibited the oxidative damage of three protein models induced by alcoxyl radical in a dose-dependent manner. Furthermore, WLP inhibited the growth of SGC-7901 cells in time- and concentration-dependent manner. In conclusion, WLP show significant inhibitory effect on protein oxidative damage induced by hydroxyl radical and alcoxyl radical as well as anti-proliferation capacity on SGC-7901 cells.

American Journal of Biochemistry and Biotechnology
Volume 16 No. 2, 2020, 199-207

DOI: https://doi.org/10.3844/ajbbsp.2020.199.207

Submitted On: 30 December 2019 Published On: 26 April 2020

How to Cite: Yang, S., Xie, Z., Yu, S., He, M., Liu, Q., Xiao, H. & Song, Y. (2020). Inhibitory Effect of Crude Walnut Leaf Polysaccharides on Oxidative Damage of Proteins and Proliferation of SGC-7901 Cells. American Journal of Biochemistry and Biotechnology, 16(2), 199-207. https://doi.org/10.3844/ajbbsp.2020.199.207

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Keywords

  • Walnut Leaf
  • Polysaccharide
  • Protein
  • Oxidative Damage
  • SGC-7901 Cells