Research Article Open Access

Improved SDA Production in High Lipid Accumulating Strain of Mucor circinelloides WJ11 by Genetic Modification

Junhuan Yang1, Md. Ahsanul Kabir Khan1, Sergio López-García2, Shaista Nosheen1, Yusuf Nazir1, Huaiyuan Zhang1, Victoriano Garre2 and Yuanda Song1
  • 1 Shandong University of Technology, China
  • 2 Universidad de Murcia, Spain

Abstract

Stearidonic Acid (SDA; 18:4, n-3) is a ω-3 polyunsaturated fatty acid which is nutritionally important and has pharmaceutical applications. Hence, scientists are trying to construct SDA producing oleaginous microorganisms by genetic modification. Two enzymes, Delta-6 Desaturase (D6D) and ω-3 desaturase catalyze the reactions to produce SDA from Linoleic Acid (LA; 18:2, n-6). But the key enzyme, ω-3 desaturase converts LA to ALA and GLA to SDA is absent in high lipid accumulating Mucor circinelloides WJ11, retarding its ability to produce SDA. Therefore, in this study, we overexpressed fad3 gene from Arabidopsis thaliana in M. circinelloides WJ11 to produce SDA. Overexpression of fad3 gene in M. circinelloides WJ11 resulted in the production of 340 mg/l SDA. This research opened a new opportunity to make use of this fungus for industrial production of SDA.

American Journal of Biochemistry and Biotechnology
Volume 16 No. 1, 2020, 138-147

DOI: https://doi.org/10.3844/ajbbsp.2020.138.147

Submitted On: 17 January 2020 Published On: 23 March 2020

How to Cite: Yang, J., Khan, M. A. K., López-García, S., Nosheen, S., Nazir, Y., Zhang, H., Garre, V. & Song, Y. (2020). Improved SDA Production in High Lipid Accumulating Strain of Mucor circinelloides WJ11 by Genetic Modification. American Journal of Biochemistry and Biotechnology, 16(1), 138-147. https://doi.org/10.3844/ajbbsp.2020.138.147

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Keywords

  • Fatty Acid Desaturase 3
  • SDA Production
  • Homologous Overexpression
  • Mucor circinelloides