Improved SDA Production in High Lipid Accumulating Strain of Mucor circinelloides WJ11 by Genetic Modification
- 1 Shandong University of Technology, China
- 2 Universidad de Murcia, Spain
Abstract
Stearidonic Acid (SDA; 18:4, n-3) is a ω-3 polyunsaturated fatty acid which is nutritionally important and has pharmaceutical applications. Hence, scientists are trying to construct SDA producing oleaginous microorganisms by genetic modification. Two enzymes, Delta-6 Desaturase (D6D) and ω-3 desaturase catalyze the reactions to produce SDA from Linoleic Acid (LA; 18:2, n-6). But the key enzyme, ω-3 desaturase converts LA to ALA and GLA to SDA is absent in high lipid accumulating Mucor circinelloides WJ11, retarding its ability to produce SDA. Therefore, in this study, we overexpressed fad3 gene from Arabidopsis thaliana in M. circinelloides WJ11 to produce SDA. Overexpression of fad3 gene in M. circinelloides WJ11 resulted in the production of 340 mg/l SDA. This research opened a new opportunity to make use of this fungus for industrial production of SDA.
DOI: https://doi.org/10.3844/ajbbsp.2020.138.147
Copyright: © 2020 Junhuan Yang, Md. Ahsanul Kabir Khan, Sergio López-García, Shaista Nosheen, Yusuf Nazir, Huaiyuan Zhang, Victoriano Garre and Yuanda Song. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Keywords
- Fatty Acid Desaturase 3
- SDA Production
- Homologous Overexpression
- Mucor circinelloides