Optimization of Hydrolysis Conditions for Obtaining Antithrombotic Peptides from Tenebrio Molitor Larvae
- 1 China Jiliang University, China
Abstract
Tenebrio molitor larvae was hydrolyzed by pepsin and trypsin for preparing high antithrombotic activity hydrolysate. The significant variables were selected according to the Plackett-Burman design and further optimized by the Response Surface Methodology (RSM). Four factors, including substrate concentration, pepsin digestion time, pepsin amount and trypsin amount, respectively, influenced significantly on the hydrolysis effectiveness. The best hydrolysis conditions obtained using RSM experiments are as following: substrate concentration of 19.8 mg/mL, pepsin digestion time of 1.8 h, pepsin amount of 1634 U/mL and trypsin amount of 126 U/mL, respectively. Under the optimal hydrolysis conditions, the antithrombotic activity was 82.8% predicted by the RSM model, which was basically consistent with the 80.6% obtained in the three verification experiments. Moreover, the antithrombotic activity of the hydrolyzate had no linear relationship with the degree of hydrolysis.
DOI: https://doi.org/10.3844/ajbbsp.2019.52.60
Copyright: © 2019 Fangyuan Chen, Han Jiang, Yini Gan, Wenwei Chen and Guangrong Huang. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Keywords
- Tenebrio molitor Larvae
- Enzymatic Hydrolysis
- Antithrombotic Activity
- Plackett–Burman Design
- Response Surface Methodology