Research Article Open Access

Nondestructive Assessment of Egg Freshness using a Synchronous Fluorescence Spectral Technique

Jianhu Wu1, Guifeng Li1, Yankun Peng2, Junjie Du1, Jianguo Xu1 and Gang Gao1
  • 1 Shanxi Normal University, China
  • 2 College of Engineering China Agricultural University, China

Abstract

Freshness is an important index of egg quality. In this study, a synchronous fluorescence spectral technique was employed to determine the freshness of an intact egg. Synchronous fluorescence spectra of intact eggs were acquired using a fluorescence spectrometer supported by a laboratory fluorescence acquisition device and egg freshness (Haugh Unit) was obtained using destructive methods. Eggs feature fluorescence signals were mainly concentrated in two regions: A (excitation wavelength of 290 nm over the emission wavelength range of 320-380 nm) and B (excitation wavelength range of 380-570 nm over the emission wavelength range of 610-735 nm). The two regions were selected as regions of interest, which include 2581 Excitation-Emission (Ex-Em) wavelengths; stepwise discrimination analysis was performed on the 2581 Ex-Em wavelengths to choose optimal Ex-Em wavelength combinations. A Multiple Linear Regression (MLR) prediction model was built using fluorescence signals based on the optimal Ex-Em wavelength combinations. The results revealed that the freshness of an egg could be accurately predicted with Rp2 of 0.8879 and a root mean square error estimated by validation (SEP) of 6.2896. This work demonstrates that the synchronous fluorescence spectral technique has high potential for nondestructive sensing of egg freshness.

American Journal of Biochemistry and Biotechnology
Volume 15 No. 4, 2019, 230-240

DOI: https://doi.org/10.3844/ajbbsp.2019.230.240

Submitted On: 12 September 2019 Published On: 19 December 2019

How to Cite: Wu, J., Li, G., Peng, Y., Du, J., Xu, J. & Gao, G. (2019). Nondestructive Assessment of Egg Freshness using a Synchronous Fluorescence Spectral Technique. American Journal of Biochemistry and Biotechnology, 15(4), 230-240. https://doi.org/10.3844/ajbbsp.2019.230.240

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Keywords

  • Synchronous Fluorescence Spectra
  • Eggs Freshness
  • Stepwise Discrimination Analysis
  • Multiple Linear Regressions