Research Article Open Access

Two Co-Expression Strategies for Eicosapentaenoic Acid Production in Mortierella alpina

Xin Tang1, Haiqin Chen1, Xiaoke Zhang1, Zhennan Gu1, Hao Zhang1, Yong Q. Chen1 and Wei Chen2
  • 1 Jiangnan University, China
  • 2 Beijing Technology and Business University (BTBU), China

Abstract

The omega-3 long-chain Polyunsaturated Fatty Acid (PUFA) Eicosapentaenoic Acid (EPA) has beneficial effects on human health, leading to its use in health products and foods. The oleaginous microorganism Mortierella alpina has been used in the industrial production of Arachidonic Acid (AA). The omega-3 desaturase oPpFADS17 is a key enzyme in the bioconversion of AA to EPA from Phytophthora parasitica and Glucose-6-Phosphate Dehydrogenase (G6PD2) is a critical enzyme that provide reducing power NADPH for lipid biosynthesis. In this study, we used a double expression cassette and 2A peptide strategies to co-express the these two critical enzymes. Subsequently, we investigated the effects of these strategies on total lipid and EPA accumulation. The recombination strains generated using the above-described strategies exhibited no differences in cell growth, compared with the control strain. Recombination strains generated using the double expression cassette exhibited an increase in total lipids to 43% of the cell dry weight, but did not accumulate EPA. Recombination strains generated using the 2A peptide strategy exhibited increased EPA accumulation, such that this PUFA accounted for 30% of the total lipid content. These co-expression strategies provide the improvements of multi-gene modification in PUFA accumulation in M. alpina.

American Journal of Biochemistry and Biotechnology
Volume 15 No. 4, 2019, 208-217

DOI: https://doi.org/10.3844/ajbbsp.2019.208.217

Submitted On: 5 August 2019 Published On: 5 December 2019

How to Cite: Tang, X., Chen, H., Zhang, X., Gu, Z., Zhang, H., Chen, Y. Q. & Chen, W. (2019). Two Co-Expression Strategies for Eicosapentaenoic Acid Production in Mortierella alpina. American Journal of Biochemistry and Biotechnology, 15(4), 208-217. https://doi.org/10.3844/ajbbsp.2019.208.217

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Keywords

  • Co-expression
  • Double Expression Cassette
  • 2A Peptide
  • Mortierella alpine
  • EPA