Research Article Open Access

The Contamination Degree of Characteristic Fungi in Jet Fuel Detected by ATP Bioluminescence

Zezhen Li1, Yun Xiong1, Bo Chen2 and ZhenQiao Ma3
  • 1 Amry Logistics University of PLA, China
  • 2 Sinopec Zhenhai Refining and Chemical Corps Room, China
  • 3 3Hefei Peakedness Biological Technology Co., China

Abstract

Adenosine Triphosphate (ATP) lysate of the characteristic fungi in jet fuel was screened and three fluorescein-luciferase systems were compared in the paper. The correlation between ATP bioluminescence and traditional plate count for measuring the number of microorganism was also investigated and then a method of detecting the contamination degree of the characteristic fungi of jet fuel by ATP bioluminescence were initially established. The results show that the effect of ATP extraction taking surfactant Benzyldimethylhexadecylammonium chloride (BAC) as the microbial lysate was optimal and the optimal concentration and the action time were 0.15% and 30s respectively; The fluorescein-luciferase system after screening has a good detection limit, up to 10-15mol ATP; using ATP bioluminescence and traditional plate method to measure the microbial quantity, they have good correlation. Utilizing ATP bioluminescence to detect the contamination degree of the characteristic fungi in jet fuel can shorten the test time to 10min, which is suitable for rapid detection and has good application prospects.

American Journal of Biochemistry and Biotechnology
Volume 14 No. 2, 2018, 162-167

DOI: https://doi.org/10.3844/ajbbsp.2018.162.167

Submitted On: 18 April 2018 Published On: 28 May 2018

How to Cite: Li, Z., Xiong, Y., Chen, B. & Ma, Z. (2018). The Contamination Degree of Characteristic Fungi in Jet Fuel Detected by ATP Bioluminescence. American Journal of Biochemistry and Biotechnology, 14(2), 162-167. https://doi.org/10.3844/ajbbsp.2018.162.167

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Keywords

  • ATP Bioluminescence
  • Jet Fuel
  • Microbial Contamination
  • Lysate