Research Article Open Access

The -863 TNF-α Polymorphism and Primary Open Angle Glaucoma in Egyptians

Walaa Bayoumie Abdulwahhab El Gazzar1, Shaymaa Mohamed Abd El Rahman1, Mohamed Nagy Elmohamady1, Ahmed Mohamed Saeed1 and Usama Shalaby1
  • 1 Benha University, Egypt

Abstract

Growing evidence indicates that TNF-alpha is involved in the pathogenesis of POAG in several ways, primarly by induction of retinal ganglion cells apoptosis and therefore optic nerve degeneration. TNF-alpha and POAG relationship has been studied at the genetic level with variable results in different populations. The transcription rate and the release of the TNF-alpha cytokine have been reported to be affected by polymorphisms in the promoter of the TNF- alpha gene. Polymorphisms at positions -238 and -308 are the most frequent studied. Another polymorphism, at the position -863 in the promoter region, has been less studied, but a homozygous AA allele appears protective in a Chinese population. Our aim was to assess the potential association of -863C/A TNF-alpha gene promoter polymorphisms with POAG in an Egyptian group of subjects. Genotyping of the TNF-alpha (-863) polymorphism was done for 228 POAG patients and 230 control subjects using the PCR-based, Restriction Fragment Length Polymorphism (RFLP) assay. TNF-alpha (-863) A/A genotype was absent in both groups. There was no significant difference between both groups as regards to TNF-α (-863) A allele carriage (6.14 versus 10.43%; p = 0. 099)). Also the genotype TNF-α (-863) C/C and the frequency of the tumor necrosis factor-alpha (-863) C allele did not significantly differ between both groups (93.86 versus 89.57%; p = 0. 099) and 96.93 versus 94.78%; p = 0. 107) respectively. Our data indicated that the TNF-alpha (-863) a allele is not linked with primary open angle glaucoma protection among Egyptian patients.

American Journal of Biochemistry and Biotechnology
Volume 13 No. 2, 2017, 85-89

DOI: https://doi.org/10.3844/ajbbsp.2017.85.89

Submitted On: 31 March 2017 Published On: 1 June 2017

How to Cite: El Gazzar, W. B. A., El Rahman, S. M. A., Elmohamady, M. N., Saeed, A. M. & Shalaby, U. (2017). The -863 TNF-α Polymorphism and Primary Open Angle Glaucoma in Egyptians. American Journal of Biochemistry and Biotechnology, 13(2), 85-89. https://doi.org/10.3844/ajbbsp.2017.85.89

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Keywords

  • Polymorphism
  • PCR-RFLP
  • POAG
  • TNF-α
  • Glaucoma