Research Article Open Access

Isolation, Identification and Genomic Analysis of Plesiomonas shigelloides Isolated from Diseased Percocypris pingi (Tchang, 1930)

Lei Pan1, Shuiyi Liu2, Xuwei Cheng1, Yiting Tao1, Tao Yang3, Peipei Li3, Zhengxiang Wang1, Dongguo Shao4 and Defeng Zhang5
  • 1 Hubei University, China
  • 2 Huazhong University of Science and Technology, China
  • 3 Wuhan Heyuan Green Biological Co., Ltd., China
  • 4 Wuhan University, China
  • 5 Pearl River Fisheries Research Institute, China

Abstract

Recently, the outbreak of a serious infectious disease of unknown etiology was noted in Percocypris pingi (Tchang, 1930) farms in Yunnan province. Due to currently limited information, we aimed to identify the pathogen isolates, determine the susceptibility of the isolates, evaluate the pathogenicity and analyze the genome of the representative strain. Ten strains of Gram-negative rods were isolated from diseased P. pingi and the isolates were identified as Plesiomonas shigelloides based on biochemical characteristics, 16S rRNA gene sequencing and species-specific PCR detection. The results of susceptibility analysis showed that two selected strains LS1 and LL2 were resistant to ampicillin, penicillin G, trimethoprim/sulfamethoxazole, tetracycline, enrofloxacin, nalidixic acid and enoxacin. A virulence assay indicated that the pathogen was virulent to zebrafish. Genomic analysis revealed that the LS1 isolate was closely related to strain GN7, which was isolated from animal farms. To the best of our knowledge, this is the first report of P. shigelloides as a pathogen of P. pingi. This study will provide a rational framework for exploration of epidemiological analysis of P. shigelloides in fish diseases and would further benefit conservation of the species.

American Journal of Biochemistry and Biotechnology
Volume 13 No. 4, 2017, 226-232

DOI: https://doi.org/10.3844/ajbbsp.2017.226.232

Submitted On: 25 October 2017 Published On: 22 December 2017

How to Cite: Pan, L., Liu, S., Cheng, X., Tao, Y., Yang, T., Li, P., Wang, Z., Shao, D. & Zhang, D. (2017). Isolation, Identification and Genomic Analysis of Plesiomonas shigelloides Isolated from Diseased Percocypris pingi (Tchang, 1930). American Journal of Biochemistry and Biotechnology, 13(4), 226-232. https://doi.org/10.3844/ajbbsp.2017.226.232

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Keywords

  • Plesiomonas shigelloides
  • Percocypris pingi (Tchang, 1930)
  • Identification
  • Genomic Analysis