Research Article Open Access

Separation and Identification of Two Fungal Strains in Stored Maize

Xiaoyu Yue1, Lynne McLandsborough1, Jian Zou2 and Xueqin Gao2
  • 1 University of Massachusetts, United States
  • 2 Henan University of Animal Husbandry and Economy, China

Abstract

The microbiological spoilage of stored grain is important for human health and economic loss. Two major predominant grain spoilage fungi (M15 and M16) were isolated from maize from a granary in China. Morphological observation of the fungal isolates, analysis of their ITS sequences and construction of their phylogenetic trees were conducted on these two strains, which were identified as Fusarium solani (M15) and Aspergillus sclerotiorum (M16). This method was shown to be a feasible, accurate and convenient way to identify fungi in stored grain.

American Journal of Biochemistry and Biotechnology
Volume 13 No. 3, 2017, 132-139

DOI: https://doi.org/10.3844/ajbbsp.2017.132.139

Submitted On: 6 July 2017 Published On: 3 August 2017

How to Cite: Yue, X., McLandsborough, L., Zou, J. & Gao, X. (2017). Separation and Identification of Two Fungal Strains in Stored Maize. American Journal of Biochemistry and Biotechnology, 13(3), 132-139. https://doi.org/10.3844/ajbbsp.2017.132.139

  • 3,538 Views
  • 1,992 Downloads
  • 1 Citations

Download

Keywords

  • Maize
  • Morphological Characteristics
  • Analysis of ITS Sequence
  • Phylogenetic Tree