Research Article Open Access

Cocoa-Butter-Equivalent Production from Yarrowia lipolytica by Optimization of Fermentation Technology

Lina Zhao1, Bo Li1, Dan Xiong2, Huaiyuan Zhang3, Xin Tang2, Hongyin Zhang1, Yuanda Song3 and Shengru Yang4
  • 1 Jiangsu University, China
  • 2 Jiangnan University, China
  • 3 Shandong University of Technology, China
  • 4 Henan University of Animal Husbandry and Economy, China

Abstract

In order to obtain the optimal control point for single-cell lipid production with fatty acid profile similar to cocoa butter, this study explored the effects of carbon source, nitrogen source, temperature, sterculic acid methyl ester, cobalt on cell growth and lipid accumulation of Yarrowia lipolytica. Glycerol and ammonium tartrate-yeast extract were chosen as carbon source and nitrogen source for production of cocoa butter equivalent. Gradual increase in temperature (20-35°C) resulted in growth reduction, while increased in lipid content per Cell Dry Weight (CDW) (10.13 to 19.7%, w/w) and Saturated Fatty Acids (SFA). Our results suggests that the optimum conditions for Y. lipolytica to synthesize cocoa butter equivalent was 30°C with 0.6 mg L-1 of CoCl2.6H2O or 0.03 mL L-1 sterculic acid methyl ester in the medium with glycerol and ammonium tartrate-yeast extract as carbon source and nitrogen source.

American Journal of Biochemistry and Biotechnology
Volume 12 No. 4, 2016, 196-205

DOI: https://doi.org/10.3844/ajbbsp.2016.196.205

Submitted On: 14 November 2016 Published On: 29 December 2016

How to Cite: Zhao, L., Li, B., Xiong, D., Zhang, H., Tang, X., Song, Y. & Yang, S. (2016). Cocoa-Butter-Equivalent Production from Yarrowia lipolytica by Optimization of Fermentation Technology. American Journal of Biochemistry and Biotechnology, 12(4), 196-205. https://doi.org/10.3844/ajbbsp.2016.196.205

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Keywords

  • Cobalt
  • Cocoa Butter Equivalent
  • Saturated Fatty Acids
  • Sn-2 Position
  • Sterculic Acid Methyl Ester
  • Triacylglycerols