Research Article Open Access

Amino Acid Sequence of Amylase Type Alpha, MiAmy, from Ok-Rong Mango (Mangifera indica Linn. cv. Ok-Rong)

Raksmont Ubonbal1, Saijai Posoongnoen2, Jureerut Daduang1, Sompong Klaynongsruang1 and Sakda Daduang1
  • 1 Khon Kaen University, Thailand
  • 2 Nakhon Ratchasima Rajabhat University, Thailand

Abstract

The 594-amino acid residue sequence of α-amylase, MiAmy, from the Ok-rong mango (Mangifera indica Linn. cv. Ok-rong), in the ripening stage, was determined through Reverse Transcription Polymerase Chain Reaction (RT-PCR). Sequence alignments and evolutionary tree analyses revealed its high similarity to plastid α-amylase from many other plants. The sequence was revealed to have four conserved regions with catalytic amino acid residues for the active site. It was classified as a member of α-amylase family 13 because it has an active Domain A, similar to a (α/β)8-barrel structure. Three-dimensional structural predictions revealed that this partial sequence completely covered all of necessary domains for amylase activity.

American Journal of Biochemistry and Biotechnology
Volume 11 No. 3, 2015, 119-126

DOI: https://doi.org/10.3844/ajbbsp.2015.119.126

Submitted On: 10 July 2015 Published On: 19 August 2015

How to Cite: Ubonbal, R., Posoongnoen, S., Daduang, J., Klaynongsruang, S. & Daduang, S. (2015). Amino Acid Sequence of Amylase Type Alpha, MiAmy, from Ok-Rong Mango (Mangifera indica Linn. cv. Ok-Rong). American Journal of Biochemistry and Biotechnology, 11(3), 119-126. https://doi.org/10.3844/ajbbsp.2015.119.126

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Keywords

  • α-Amylase
  • Ok-Rong Mango
  • Amylase Family 13
  • Amino Acid Sequence
  • Mangifera indica