Research Article Open Access

Inhibition of Interleukin-6 Expression by Curcumin in Rat Vascular Smooth Muscle Explants In Vitro

Yanti1, Stephanie1, Yuliani1, F.G. Winarno1 and Maggy T. Suhartono2
  • 1 Atma Jaya Catholic University, Indonesia
  • 2 Bogor Agricultural University, Indonesia

Abstract

In atherosclerotic plaques, infectious agents from microbes may release Lipopolysaccharide (LPS) and heat shock proteins that can stimulate the production of mediators, i.e., pro-inflammatory cytokines, by vascular endothelial cells and Smooth Muscle Cells (SMCs). The elevated level of Interleukin-6 (IL-6) is strongly associated with the development of atherosclerosis. Here, we investigated whether curcumin isolated from the rhizome of Curcuma longa affected the expression of IL-6 at protein and gene levels in rat smooth muscle cells treated with LPS in vitro by conducting ELISA and Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) assays. LPS at 2 µg mL-1 activated the expression of IL-6 protein and mRNA in SMCs. Curcumin (1-15 µM) caused the decreased levels of IL-6 protein and mRNA in the dose-dependent manner in LPS-induced SMCs, indicating its potential antiatherosclerotic effect for cardiovascular risk management. In addition, curcumin also partially blocked the activation of LPS-induced phosphorylation of MAP kinases, i.e., ERK1/2, p38 and JNK, in SMCs, suggesting it may inhibit IL-6 expression via attenuating MAP kinase signaling pathways in LPS-induced SMCs. These data may in part explain the molecular action of antiatherosclerotic effects of curcumin.

American Journal of Biochemistry and Biotechnology
Volume 10 No. 4, 2014, 260-266

DOI: https://doi.org/10.3844/ajbbsp.2014.260.266

Submitted On: 18 September 2014 Published On: 15 December 2014

How to Cite: Yanti, ., Stephanie, ., Yuliani, ., Winarno, F. & Suhartono, M. T. (2014). Inhibition of Interleukin-6 Expression by Curcumin in Rat Vascular Smooth Muscle Explants In Vitro. American Journal of Biochemistry and Biotechnology, 10(4), 260-266. https://doi.org/10.3844/ajbbsp.2014.260.266

  • 1,098 Views
  • 1,278 Downloads
  • 0 Citations

Download

Keywords

  • Curcumin
  • Curcuma Xanthorrhiza Roxb.
  • Interleukin-6
  • MAP Kinase
  • Rat Smooth Muscle Cells