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Frequency: Quarterly
ISSN: 1553-3468 (Print)
ISSN: 1558-6332 (Online)
Research Article Open Access

DNA-MARKERS OF SUNFLOWER RESISTANCE TO THE DOWNY MILDEW (PLASMOPARA HALSTEDII)

A. V. Usatov1, A. I. Klimenko2, K. V. Azarin1, O. F. Gorbachenko3, N. V. Markin1, V. E. Tikhobaeva1, Yu. A. Kolosov2, O. A. Usatova1, S. Yu. Bakoev2, M. Yu. Bibov1 and L. V. Getmantseva2
  • 1 Southern Federal University, Russia
  • 2 Don State Agrarian University, Russia
  • 3 All-Russia Research Institute of Oil Crops by V.S. Pustovoit, Russia

Abstract

One of the areas of biotechnology sunflower is the development and testing of DNA markers of important agronomic traits and in particular markers of resistance to downy mildew. Resistance of 16 Rf-lines of sunflower to the races 330 and 710 of Plasmopara halstedii has been studied. Genotyping of these lines was carried out using 9 STS-markers of three Pl-loci, Pl5, Pl6 and Pl8, associated with the resistance of sunflower to downy mildew. Only two out of nine STS-markers, HaP2 and HaP3 (locus Pl6), allowed us to identify the lines, which demonstrated resistance to the downy mildew under the conditions of artificial infection.

American Journal of Biochemistry and Biotechnology
Volume 10 No. 2, 2014, 125-129

DOI: https://doi.org/10.3844/ajbbsp.2014.125.129

Submitted On: 30 March 2014 Published On: 24 July 2014

How to Cite: Usatov, A. V., Klimenko, A. I., Azarin, K. V., Gorbachenko, O. F., Markin, N. V., Tikhobaeva, V. E., Kolosov, Y. A., Usatova, O. A., Bakoev, S. Y., Bibov, M. Y. & Getmantseva, L. V. (2014). DNA-MARKERS OF SUNFLOWER RESISTANCE TO THE DOWNY MILDEW (PLASMOPARA HALSTEDII). American Journal of Biochemistry and Biotechnology, 10(2), 125-129. https://doi.org/10.3844/ajbbsp.2014.125.129

Open AccessCopyright: © 2014 A. V. Usatov, A. I. Klimenko, K. V. Azarin, O. F. Gorbachenko, N. V. Markin, V. E. Tikhobaeva, Yu. A. Kolosov, O. A. Usatova, S. Yu. Bakoev, M. Yu. Bibov and L. V. Getmantseva. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Keywords

  • Downy Mildew
  • STS-Markers
  • Marker-Assisted Selection
  • Sunflower