Research Article Open Access

Protective Effects of Propolis on Gamma-Irradiated Nigella sativa Extract Induced Blood and Immune Changes in Wistar Rats

Osama Moseilhy Saleh1, Mohamed Mohamed Soliman1, Ahmed Abdel-Khalik Mansour2 and Omniya Mahmoud Abdel-Hamid3
  • 1 Taif University, Saudi Arabia
  • 2 Ain Shams University, Egypt
  • 3 Benha University, Egypt

Abstract

The present study conducted to test the effect of Nigella Sativa (NS, 5 mg kg-1 of body weight), or γ-irradiated Nigella Sativa (GRNS) on the changes of blood component profiles, liver, kidney functions and immune cytokines secretion in male Wistar rats. Moreover, the possible protection by propolis (200 mg kg-1 B. W.) on the changes induced by NS and GRNS was examined. Results revealed that both NS and GRNS administration for two weeks induced changes in blood, GPT, GOT and urea levels and co-administration with propolis significantly ameliorated such changes. Also, liver histology showed numerous vacuolar degeneration and fatty changes in γ-irradiated groups which disappeared in presence of propolis. Kidney histology of NS administered rats showed less lymphocytic infiltration, while GRNS groups showed desquamation in the cytoplasm of the renal tubules, hemorrhage in the renal corpuscle and lymphocytic infiltration which disappeared when propolis given together with GRNS. Finally propolis induced protective effect on the changes induced in TNF-α and IL-10 secretion by either NS or GRNS in Wistar rats. In conclusion, the findings of present study clarified the protective effect of propolis on changes induced by γ-irradiated NS on blood, liver, kidney and cytokines changes in Wistar rats.

American Journal of Biochemistry and Biotechnology
Volume 9 No. 2, 2013, 162-171

DOI: https://doi.org/10.3844/ajbbsp.2013.162.171

Submitted On: 29 April 2013 Published On: 20 June 2013

How to Cite: Saleh, O. M., Soliman, M. M., Mansour, A. A. & Abdel-Hamid, O. M. (2013). Protective Effects of Propolis on Gamma-Irradiated Nigella sativa Extract Induced Blood and Immune Changes in Wistar Rats. American Journal of Biochemistry and Biotechnology, 9(2), 162-171. https://doi.org/10.3844/ajbbsp.2013.162.171

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Keywords

  • Blood Changes
  • γ-Irradiation
  • Nigella Sativa
  • Propolis
  • Wistar Rats