Research Article Open Access

Integrated Supercritical Fluid Extraction and Bioprocessing

Owen Catchpole1, Stephen Tallon1, Peter Dyer1, Fernando Montanes1, Teresa Moreno1, Erika Vagi1, Wayne Eltringham1 and Jagan Billakanti1
  • 1 Industrial Research Limited, New Zealand


Supercritical fluids are increasingly being used and promoted at a laboratory and pilot scale to produce high value, natural bioactives from biologically based raw materials. Supercritical CO2 is overwhelmingly the solvent of choice for these operations, but is largely limited to the processing of dry raw materials and the extraction of low polarity, low molecular weight compounds. The use of co-solvents and the use of alternative ‘near-critical’ extraction fluids such as dimethyl ether show potential to mitigate these limitations. Commercialisation of new supercritical extraction processes has arguably been limited because the supercritical extraction process has been developed in isolation of other processing steps necessary to achieve a successful product. This study reviews recent developments in integrated processing that incorporate the use of supercritical fluids for bioseparations and in particular process schemes that produce high value natural bioactives. Integrated processes include prior operation (fermentation, extraction, enzyme pre-treatment, physical fractionation or size reduction) followed by supercritical extraction or fractionation and processes in which operations are carried out in situ in supercritical fluids (supercritical chromatography, enzymatic conversion, precipitation and coating of solutes). The use of co-solvents and alternative extraction solvents in these processes is discussed. Prospects for future developments are also discussed.

American Journal of Biochemistry and Biotechnology
Volume 8 No. 4, 2012, 263-287


Submitted On: 23 October 2012 Published On: 25 December 2012

How to Cite: Catchpole, O., Tallon, S., Dyer, P., Montanes, F., Moreno, T., Vagi, E., Eltringham, W. & Billakanti, J. (2012). Integrated Supercritical Fluid Extraction and Bioprocessing. American Journal of Biochemistry and Biotechnology, 8(4), 263-287.

  • 45 Citations



  • Supercritical Fluid
  • Extraction
  • Fractionation
  • Fermentation
  • Enzymatic Conversion
  • Integrated Processing