Research Article Open Access

Potential Applications of Chitosan Nanoparticles as Novel Support in Enzyme Immobilization

Hoda Jafarizadeh Malmiri1, Mohammad Ali Ghaz Jahanian1 and Aydin Berenjian2
  • 1 Sahand University of Technology, Iran
  • 2 The University of Sydney, Australia


Chitosan is an attractive natural biopolymer from renewable resources with the presence of reactive amino and hydroxyl functional groups in its structure. Due to the good biocompatibility of chitosan, it can be used in magnetic-field assisted drug delivery, enzyme or cell immobilization and many other industrial applications. In the past decade, nanotechnology has been a considerable research interest in the area of preparation of immobilized enzyme carriers. This study looks at characteristics and applications of chitosan and chitosan nanoparticles and their potentials as suitable supports for enzyme immobilization. Results indicated that activity of immobilized enzymes and performance of enzyme immobilization onto chitosan nanoparticles are higher than chitosan macro and microparticles. As compared to other biopolymers nanoparticles, application of chitosan nanoparticles to immobilize enzymes strongly increases stability of immobilized enzymes and their easy separability from the reaction mixture at the end of the biochemical process.

American Journal of Biochemistry and Biotechnology
Volume 8 No. 4, 2012, 203-219


Submitted On: 3 September 2012 Published On: 25 December 2012

How to Cite: Malmiri, H. J., Jahanian, M. A. G. & Berenjian, A. (2012). Potential Applications of Chitosan Nanoparticles as Novel Support in Enzyme Immobilization. American Journal of Biochemistry and Biotechnology, 8(4), 203-219.

  • 141 Citations



  • Chitosan
  • Chitosan Nanoparticles
  • Immobilized Enzyme
  • Support
  • Enzyme Activity
  • Stability