Research Article Open Access

Uterus-Relaxing Study of a Sudanese Herb (El-Hazha)

Aimun Abdelgaffar Elhassan Ahmed1, Robert Gaspar1, Arpad Marki1, Andrea Vasas2, Mahmoud Mudawi Eltahir Mudawi3, Judit Hohmann3 and George Falkay1
  • 1 University of Szeged, Hungary
  • 2 Omdurman Islamic University, Sudan
  • 3 University of Szeged, Sudan

Abstract

Problem statement: The aim of this study is to investigate the pharmacological effects of the Methanolic-extract (AH2) of El-Hazha and its sub-fractions. Approach: These investigations were carried out on in vitro isolated uterus preparations from Non-Pregnant (NP) and Late-Pregnant rats (LP). In parallel displacement radio-ligand binding assay was performed for β-Adrenergic Receptors (β-ADR). Results: Showed that the herb and its different fractions produced dose-dependent relaxant effect (p<0.05, t-test, n = 6) on uterine contraction elicit by 25 mM KCl in both NP and LP uteri that affected significantly by fractionation, however, the effect of the most active fraction (AH2-11) was reversed by prior addition of propranolol (non-specific β-antagonist), but not affected by progesterone pre treatment of the LP rats. In addition, AH2 only in high concentration displaced isotopes from β-ADR, this affinity changed markedly by fractionation. Conclusion: We validate the fractionation effect on its relaxant activity and found partial role for β-ADR on mediating this activity. Future study was recommended to isolate and investigate its active components to enhance this activity or to discover a new novel natural therapeutic agent(s).

American Journal of Biochemistry and Biotechnology
Volume 6 No. 3, 2010, 231-238

DOI: https://doi.org/10.3844/ajbbsp.2010.231.238

Submitted On: 4 November 2010 Published On: 30 September 2010

How to Cite: Ahmed, A. A. E., Gaspar, R., Marki, A., Vasas, A., Mudawi, M. M. E., Hohmann, J. & Falkay, G. (2010). Uterus-Relaxing Study of a Sudanese Herb (El-Hazha). American Journal of Biochemistry and Biotechnology, 6(3), 231-238. https://doi.org/10.3844/ajbbsp.2010.231.238

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Keywords

  • Haplophylium tuberculatum
  • radio-ligand binding assay
  • β-Adrenergic Receptors (β-ADR)
  • Non-Pregnant (NP)
  • Late-Pregnant rats (LP)
  • Sudanese plant
  • charles-river laboratories
  • medicinal and aromatic plants
  • tocolytic agent
  • pharmacological effects
  • progesterone treatment
  • uterus-relaxing study