Research Article Open Access

The Effect of Citrullus colocynthis Pulp Extract on the Liver of Diabetic Rats a Light and Scanning Electron Microscopic Study

Mohammad Khalil1, Gamal Mohamed1, Mohammad Dallak1, Fahaid Al-Hashem1, Hussein Sakr2, Refaat A. Eid1, Mohamed A. Adly1, Mahmoud Al-Khateeb3, Saleh Banihani3, Zuhair Hassan3 and Nabil Bashir3
  • 1 King Khalid University, Egypt
  • 2 Electron Microscope Unit, Egypt
  • 3 Jordan University of Science and Technology, Egypt


Problem statement: The goal of the current investigation was to clarify the effects of Citrullus colocynthis pulp extract on the structure of the liver of diabetic rats at both light and scanning electron microscopic levels. Approach: Forty-eight adult male albino rats were equally allocated into four groups: Group1: control, Group 2: Citrullus colocynthis-treated, Group 3: diabetic rats and Group4: diabetic rats treated with Citrullus colocynthis. All treatments were administered via an intragastric tube. Diabetes was induced in the rats of groups 3 and 4 by an intraperitoneal injection with alloxan. Results: The liver of Citrullus colocynthis-treated rats revealed minor histological changes versus the control animals. In group 3 animals, diabetes caused degenerative alterations in the form of disorganization of the hepatic cords, cytoplasmic vacuolization and pyknosis of the nuclei of hepatocytes and inflammatory cell infiltration. Scanning electron microscope examination of these livers revealed numerous lipid droplets within hepatocytes, damaged blood sinusoids and hemorrhage of erythrocytes between hepatocytes and inside Disse's spaces. On the other hand, the normal histological and scanning ultrastructural features were nearly resumed in the liver of diabetic rats treated with Citrullus colocynthis pulp extract. Conclusion: The present study proved a lessening effect of Citrullus colocynthis pulp extract on the liver of diabetic rats. In light of these advantageous influences, it is advisable to widen the scale of its use in a trial to alleviate the diabetic hepatic adverse effects.

American Journal of Biochemistry and Biotechnology
Volume 6 No. 3, 2010, 155-163


Submitted On: 5 August 2010 Published On: 30 September 2010

How to Cite: Khalil, M., Mohamed, G., Dallak, M., Al-Hashem, F., Sakr, H., Eid, R. A., Adly, M. A., Al-Khateeb, M., Banihani, S., Hassan, Z. & Bashir, N. (2010). The Effect of Citrullus colocynthis Pulp Extract on the Liver of Diabetic Rats a Light and Scanning Electron Microscopic Study. American Journal of Biochemistry and Biotechnology, 6(3), 155-163.

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  • Citrullus colocynthis
  • liver
  • alloxan
  • diabetes
  • rats