Research Article Open Access

An Optimized Medium for Screening of L-Asparaginase production by Escherichia coli

Younes Ghasemi1, Alireza Ebrahiminezhad1, Sara Rasoul-Amini1, Gholamreza Zarrini2, Mohammad Bagher Ghoshoon2, Mohammad Javad Raee2, Mohammad Hossein Morowvat2, Farshid Kafilzadeh2 and Aboozar Kazemi2
  • 1 Shiraz University of Medical Sciences, Iran
  • 2 University of Tabriz, Iran

Abstract

Purified L-asparaginase II from Escherichia coli has been supplied and employed in the acute leukemia and other malignant neoplasms chemotherapy. L-asparaginase II gene (ansB) in E. coli is under regulation and certain conditions is needed for expression of this gene. In this investigation ,the various concentrations of modified M9 medium ingredients and various carbon source were tested to optimize the medium for expression and identification of L-asparaginase in E. coli. Finally a semi-quantitative plate assay for L-asparaginase producing Escherichia coli is reported.

American Journal of Biochemistry and Biotechnology
Volume 4 No. 4, 2008, 422-424

DOI: https://doi.org/10.3844/ajbbsp.2008.422.424

Submitted On: 12 November 2008 Published On: 31 December 2008

How to Cite: Ghasemi, Y., Ebrahiminezhad, A., Rasoul-Amini, S., Zarrini, G., Ghoshoon, M. B., Raee, M. J., Morowvat, M. H., Kafilzadeh, F. & Kazemi, A. (2008). An Optimized Medium for Screening of L-Asparaginase production by Escherichia coli. American Journal of Biochemistry and Biotechnology, 4(4), 422-424. https://doi.org/10.3844/ajbbsp.2008.422.424

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Keywords

  • L-asparaginase
  • E. coli
  • medium
  • ansB