Research Article Open Access

The Effect of Molecular Chaperone, Alpha-Crystallin, on the Heat-Induced Aggregation of Beta-lactoglobulin

Arezou Ghahghaei1
  • 1 University of Sistan and Baluchestan, Iran

Abstract

Aggregation of beta-lactoglobulin occurs mainly via intermolecular disulphide bond exchange. Upon heating, beta-lactoglobulin aggregated which increased with increasing pH. The presence of DTT led to more rapid aggregation and precipitation of beta-lactoglobulin. Alpha-Crystallin prevented the aggregation of heat-stressed beta-lactoglobulin and was a more efficient chaperone at higher pH values. In the presence of DTT, however, alpha-crystallin was a less efficient chaperone due to faster aggregation of heated and reduced beta-lactoglobulin.

American Journal of Biochemistry and Biotechnology
Volume 4 No. 4, 2008, 317-324

DOI: https://doi.org/10.3844/ajbbsp.2008.317.324

Submitted On: 3 May 2008 Published On: 31 December 2008

How to Cite: Ghahghaei, A. (2008). The Effect of Molecular Chaperone, Alpha-Crystallin, on the Heat-Induced Aggregation of Beta-lactoglobulin. American Journal of Biochemistry and Biotechnology, 4(4), 317-324. https://doi.org/10.3844/ajbbsp.2008.317.324

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Keywords

  • Beta-lactoglobulin
  • alpha-crystallin
  • crowding agent