Research Article Open Access

Proteomics Approach for Investigating the Disease Resistance Using Cucumber as Model Plant

Saligrama A. Deepak, Junko Shibato, Hideo Ishii, Yoko Ogawa, Yasukazu Yoshida, Hitoshi Iwahashi, Yoshinori Masuo, Ganesh Kumar Agrawal and Randeep Rakwal

Abstract

The profile of proteins induced in cucumber leaves pretreated with Acibenzolar-S-Methyl (ASM) and challenge inoculated with Colletotrichum orbiculare are presented. ASM was applied to the first leaf and the third leaf collected at different time intervals of 0-48 h and the total protein extracted using trichloroacetic acid acetone was analyzed by two-dimensional gel electrophoresis. Increased expressions of pyridoxine biosynthesis protein, carbonic anhydrase, proteasome subunit (beta type 1), phosphomannomutase and ascorbate peroxidase proteins were noticed in cucumber plants systemically induced resistance by ASM. All the above proteins are well known to play a major role in scavenging of active oxygen species and preventing the cell from oxidative damage. Our results suggest that production and scavenging of ROS are one of the major events that occur during ASM induced systemic resistance in cucumber.

American Journal of Biochemistry and Biotechnology
Volume 4 No. 3, 2008, 231-238

DOI: https://doi.org/10.3844/ajbbsp.2008.231.238

Submitted On: 25 September 2006 Published On: 30 September 2008

How to Cite: Deepak, S. A., Shibato, J., Ishii, H., Ogawa, Y., Yoshida, Y., Iwahashi, H., Masuo, Y., Agrawal, G. K. & Rakwal, R. (2008). Proteomics Approach for Investigating the Disease Resistance Using Cucumber as Model Plant. American Journal of Biochemistry and Biotechnology, 4(3), 231-238. https://doi.org/10.3844/ajbbsp.2008.231.238

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Keywords

  • Cucumber
  • lysis buffer
  • mass spectrometry
  • trichloroacetic acid/acetone
  • two-dimensional gel electrophoresis