Research Article Open Access

New Bacterial Species Isolated from Malaysian Sea Cucumbers with Optimized Secreted Antibacterial Activity

Abd El-Aziem Farouk1, Faizal Abd. Hamid Ghouse1 and B.H. Ridzwan1
  • 1 International Islamic University Malaysia Jalan Gombak, Malaysia

Abstract

In this study, several Malaysian sea cucumber species that have traditional medicine value were selected and from them, the native bacterial population was isolated. Optimization of growth was designed and all bacterial secretions were tested for antibacterial properties. 30 bacterial types were isolated and 7 types recorded moderate antibacterial activity against K. pneumoniae, S. marscens, P. aeruginosa and E. feacalis. Antibacterial plate screening was done, with various testing parameters. Turbidometry revealed a single dose of the 10x concentrated crude antibacterial extracts were effective in preventing pathogenic growth for up to 4 hrs. PCR and subsequent sequencing of the 16S rDNA showed that the bacterial species were from the halopholic Bacillus and Klebsiella genera.

American Journal of Biochemistry and Biotechnology
Volume 3 No. 2, 2007, 60-65

DOI: https://doi.org/10.3844/ajbbsp.2007.60.65

Submitted On: 25 September 2006 Published On: 30 June 2007

How to Cite: Farouk, A. E., Ghouse, F. A. H. & Ridzwan, B. (2007). New Bacterial Species Isolated from Malaysian Sea Cucumbers with Optimized Secreted Antibacterial Activity. American Journal of Biochemistry and Biotechnology, 3(2), 60-65. https://doi.org/10.3844/ajbbsp.2007.60.65

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Keywords

  • Sea cucumber bacteria
  • antibacterial peptide
  • antibacterial screening
  • turbidometry and spectrophotometry
  • 16S rDNA