Research Article Open Access

Multiple Antibiotic Resistances of Vibrio Isolates from Coastal and Brackish Water Areas

S. Manjusha1, G. B. Sarita1, K. K. Elyas1 and M. Chandrasekaran1
  • 1 Cochin University of Science and Technology, India

Abstract

An experiment was designed to assess the occurrence of multiple antibiotic resistances in Vibrio spp. from different (brackish and marine) environments. Water samples from nine marine landing sites and two coastal inland aquaculture farms were screened for the Vibrio spp. and assessed their resistance to twenty-two different antibiotics, which are commonly encountered in the aquatic ecosystem. Tissue samples (shrimp, mussel and sepia) were tested from the sampling site with highest antibiotic resistance. Of the total 119 Vibrio isolates, 16. 8% were susceptible to all antibiotics. Of the resistant (83.19%) Vibrio strains, 30.3% were resistant against three antibiotics, 55.5% were resistant against 4-10 antibiotics, 14.14% were resistant against more than 10 antibiotics and 54% have shown multiple antibiotics resistance (MAR). Antibiotic resistance index was higher in Coastal 3, 6, Aqua farm 2 in isolates from water samples and all the tissues tested. Interestingly, incidence of antibiotic resistance in isolates from water samples was comparatively lower in aquaculture farms than that observed in coastal areas. Highest incidence of antibiotic resistance was evident against Amoxycillin, Ampicillin, Carbencillin and Cefuroxime followed by Rifampicin and Streptomycin and lowest against Chloramphenicol, Tetracycline, Chlortetracycline, Furazolidone, Nalidixic acid, Gentamycin Sulphafurazole, Trimethoprim, Neomycin and Amikacin irrespective of the sampling sites. Results from various tissue samples collected from the sites of highest antibiotic resistance indicated that antibiotic resistance Vibrio spp. collected from fish and tissue samples were higher than that of water samples. Overall results indicated that persistent use of antibiotics against diseases in human beings and other life forms may pollute the aquatic system and their impact on developing antibiotic resistant Vibrio spp. may be a serious threat in addition to the use of antibiotics in aquaculture farms.

American Journal of Biochemistry and Biotechnology
Volume 1 No. 4, 2005, 193-198

DOI: https://doi.org/10.3844/ajbbsp.2005.193.198

Submitted On: 20 February 2006 Published On: 31 December 2005

How to Cite: Manjusha, S., Sarita, G. B., Elyas, K. K. & Chandrasekaran, M. (2005). Multiple Antibiotic Resistances of Vibrio Isolates from Coastal and Brackish Water Areas. American Journal of Biochemistry and Biotechnology, 1(4), 193-198. https://doi.org/10.3844/ajbbsp.2005.193.198

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Keywords

  • Multiple antibiotic resistance
  • Vibrio spp.
  • water
  • shrimp
  • mussel
  • sepia