Research Article Open Access

Effect of UV-C on Drought Tolerance of Alfalfa (Medicago sativa) Callus

Ali Akbar Ehsanpour1 and Roya Razavizadeh1
  • 1 Isfahan University, Iran


Water stress and unfavorable climate decrease the growth and development of plant globally. Medicago is a valuable plant as a source of food for animal, foliage and for medicine. Since, Iran is located in a dry area; consequently, the study of plant responses to water stress is important. In this research UV-C radiation was used to induce physiological and genetic changes in alfalfa callus. In vitro grown calluses of M. sativa were exposed to UV-C for 0, 15, 30 and 60 min and then, were transferred to MS medium containing 0, 2, 5, 10, 20, 30% PEG. All explants were kept in the dark in the culture room. Calluses tolerate to osmotic stress were selected according to fresh weight. Results showed that radiation of UV-C for 60 min increased the osmotic tolerance and decreased the effect of drought stress.

American Journal of Biochemistry and Biotechnology
Volume 1 No. 2, 2005, 107-110


Submitted On: 6 July 2005 Published On: 30 June 2005

How to Cite: Ehsanpour, A. A. & Razavizadeh, R. (2005). Effect of UV-C on Drought Tolerance of Alfalfa (Medicago sativa) Callus. American Journal of Biochemistry and Biotechnology, 1(2), 107-110.

  • 22 Citations



  • Callus
  • Medicago sativa
  • UV-C
  • PEG
  • drought stress