New Bacterial Species Isolated from Malaysian Sea Cucumbers with Optimized Secreted Antibacterial Activity

Abstract

In this study, several Malaysian sea cucumber species that have traditional medicine value were selected and from them, the native bacterial population was isolated. Optimization of growth was designed and all bacterial secretions were tested for antibacterial properties. 30 bacterial types were isolated and 7 types recorded moderate antibacterial activity against K. pneumoniae, S. marscens, P. aeruginosa and E. feacalis. Antibacterial plate screening was done, with various testing parameters. Turbidometry revealed a single dose of the 10x concentrated crude antibacterial extracts were effective in preventing pathogenic growth for up to 4 hrs. PCR and subsequent sequencing of the 16S rDNA showed that the bacterial species were from the halopholic Bacillus and Klebsiella genera.