OnLine Journal of Biological Sciences

Screening of Blast Resistance Genes from South Tapanuli Rice Cultivars, North Sumatra, Indonesia

Saleha Hannum, Hesti Wahyuningsih, Riyanto Sinaga, Ummu Kulsum Hasibuan and Adrian Hartanto

DOI : 10.3844/ojbsci.2020.99.106

OnLine Journal of Biological Sciences

Volume 20, Issue 2

Pages 99-106

Abstract

Rice blast disease caused by a phytopathogenic fungus, Magnaporthe oryzae, has been considered as a major threat to global rice productivity, including Indonesia. South Tapanuli is a regency in North Sumatra, known for its intensive and significant rice production activities. A collection of 13 rice cultivars originating from the region, is claimed for its resistances against the rice blast disease based on the practices by the local farmers. Molecular screening of blast resistance (R) genes was initiated to the 13 accessions using the Polymerase Chain Reaction (PCR) targeting five blast resistance (R) genes, Pup1, Pi-37, Pi-d2, Pi-ta2, Pib and Pi-kh. Out of the 13 accessions, the Pi-d2, Pi-ta2 and Pib were detected in all cultivars, showing 100% of R gene frequency. The second dominant, Pup1 and Pi-37 gene frequencies were 76.9 and 61.5%, respectively while the least dominant, Pi-kh gene frequency was 15.4%. The number of R genes detected among cultivars was ranged between 3 to 6 genes. The genetic associations among thirteen germplasms were determined using unweighted pair group method with arithmetic mean (UPGMA) analysis. Cluster analysis revealed that the least blast-resistant cultivars: Putri Kembar, Silatian and Siganteng, were grouped into cluster 1, showing polymorphism for Pi-d2, Pi-ta2 and Pi-b. The high-resistant cultivars, Martabe-Sicondong and Sayuti, Sitolas, Pulo Raja, Pulo Pandan, Siporang and Pulo Manggis, were grouped into cluster 2 and 3, respectively. The medium blast-resistant cultivars, IR 64 and Sitampan were grouped into cluster 4, showing polymorphism for Pup1, Pi-d2, Pi-ta2 and Pi-b. These results indicated that the utilization of blast-resistant cultivars in North Tapanuli was supported by the presence of R genes.

Copyright

© 2020 Saleha Hannum, Hesti Wahyuningsih, Riyanto Sinaga, Ummu Kulsum Hasibuan and Adrian Hartanto. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.