SSR Analysis of Nuclear DNA of Annual and Perennial Sunflower Species (Helianthus L.)
N.V. Markin, A.V. Usatov, A.V. Grinko, K.F. Kan and V.A. Gavrilova
DOI : 10.3844/ojbsci.2020.77.83
OnLine Journal of Biological Sciences
Volume 20, Issue 2
Genotyping of 29 species of genus Helianthus L., including 5 annual and 24 perennial species from the collection of the N. I. Vavilov All-Russian Institute of Plant Genetic Resources has occurred, for this purpose were selected 52 SSR markers, with localization on all the 17 linkage groups of the sunflower genome. All the studied sunflower samples had unique SSR loci banding patterns. The mean PIC value varied was 0,72, which indicates the high resolution of this SSR based system for sunflower nuclear genome investigations. The discriminatory power of the marker system allowed us to classify all the sunflower species and provide the molecular barcoding. The UPGMA dendrogram, reflecting the genetic differences between 29 species of the genus Helianthus L., was constructed. Allele distribution data of the studied sunflower samples is a database that can be used to determine the levels of genetic variability, provide molecular barcoding and control the genetic integrity of collection sunflower samples.
© 2020 N.V. Markin, A.V. Usatov, A.V. Grinko, K.F. Kan and V.A. Gavrilova. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.