OnLine Journal of Biological Sciences

Antioxidant Activity, Anti-Inflammatory and Analgesic Effects of Caralluma europaea (Eddaghmouss) in Mice

Aaziz Kebbou, Mehdi Ait Laaradia, Sara Oufquir, Ayoub Aarab, Zineb El Gabbas, Hanane Rais, Abdelmajid Zyad and Abderrahman Chait

DOI : 10.3844/ojbsci.2019.272.285

OnLine Journal of Biological Sciences

Volume 19, Issue 4

Pages 272-285


The aim of the current study is to evaluate the anti-inflammatory, antioxidant and analgesic properties of ethanolic (100 and 200 mg/kg, p.o.) and ethyl acetate extracts (100 and 200 mg/kg, p.o.) of Caralluma europaea. Formalin-induced paw licking test, Acetic Acid induced Writhing Test and Hot-PlateTest were used to assess the analgesic activity. Xylene-induced ear edema test was used to evaluate anti-inflammatory activity of those extracts. In this work, the High-Performance Liquid Chromatography technique (HPLC), allowed us to identify and quantify the main phenolic compounds present in ethanolic and ethyl acetate extracts. In vitro anti-oxidant propriety was evaluated using two methods, the 2,2-Diphenyl-1Picrylhydrazyl (DPPH) radical scavenging method and reducing power methods. The main phenols identified were Catechin (24%) and quercetin (18%) in ethanolic extract, while in the ethyl acetate extract, they were quercetin (36%), P-coumaric (30%) and 2-hydroxycinnamic (25%). Analysis of our results had shown that Caralluma europaea extracts had exhibited a very potent analgesic activity. Percentage of Pain Inhibition (PPI) in the writhing test, 63.60±4.24% for the Ethanolic Extract (EE) (200 mg/kg, p.o.) and 65.39±3.27% for the Ethyl Acetate Extract (EAE). The PPI of early and late phase in the formalin test were respectively, 41% and 73% for EAE (200 mg/kg; p.o), 28% and 75% for EE. In the hotplate test, latency to the thermal stimuli was increased in a dose dependent manner after the administration of EE and EAE. However, the analgesic potential of EAE seems to be higher than EE. Both EE and EAE presented a significant in vitro redox potential and high anti-inflammatory activity. Our results have shown that Caralluma europaea is rich in phenolic compounds and possesses an important antinociceptive, anti-inflammatory and anti-oxidant activity.


© 2019 Aaziz Kebbou, Mehdi Ait Laaradia, Sara Oufquir, Ayoub Aarab, Zineb El Gabbas, Hanane Rais, Abdelmajid Zyad and Abderrahman Chait. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.