Determination of the Mutagenicity of 2-aminoanthracene Using Chicken Hepatic S-9 Fraction
Vladimir A. Chistyakov, Alexander V. Usatov, Aleksander I. Klimenko, Maria A. Kolosova, Eugenia V. Prazdnova and Alexey V. Tutel'ian
DOI : 10.3844/ojbsci.2018.442.445
OnLine Journal of Biological Sciences
Volume 18, Issue 4
The abundance of potential genotoxins makes it necessary to improve microbial test systems that allow to identify these substances quickly. At the same time, the methodology of metabolic activation of promutagens in vitro is very important. Microsomal preparations of rodents, which are generally used for that, have significant disadvantages associated with the potential health risks of the inducers of the metabolism of microsomal cytochromes. For the metabolic activation of promutagens, we have developed a protocol of microsomal homogenate fraction (S-9) preparation. We propose to complement the set of methods for quality control of feeds for valuable and rare chicken breeds with the Ames test (Salmonella/microsome) using the activated chicken hepatic S-9 homogenate fraction.
© 2018 Vladimir A. Chistyakov, Alexander V. Usatov, Aleksander I. Klimenko, Maria A. Kolosova, Eugenia V. Prazdnova and Alexey V. Tutel'ian. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.