OnLine Journal of Biological Sciences

Histone Tail Modifications of H3 and H4 during the Physarum polycephalum Cell Cycle

Marie-Claire Arnaud and Christophe Thiriet

DOI : 10.3844/ojbsci.2012.54.61

OnLine Journal of Biological Sciences

Volume 12, Issue 2

Pages 54-61


Problem statement: Histone modifications are required for chromatin activities and are believed to form an epigenetic code. However, the inheritance of the histone post-translational modifications remains unclear. Approach and Results: The aim of this study was to evaluate the histone modifications throughout the cell cycle and to determine whether the histone marks are transferred to daughter cells. Using the naturally synchronous model system Physarum polycephalum and Western blotting analyses, we showed that the histone modifications are not exclusive of the cell cycle stage. Importantly, we also demonstrated that during mitosis, a substantial fraction of the acetylation and the methylation marks of the histone H3 and H4 is removed. Conclusion: The results illustrate that a part of the histone modifications are displaced during mitosis and thus failed to exhibit an inheritance to daughter cells as proposed for the epigenetic marks.


© 2012 Marie-Claire Arnaud and Christophe Thiriet. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.