In vitro and In vivo Activity of Lactococci Strains against Helicobacter pylori
Hassina Guetarni, Ahmed Bensoltane, Djamila Aichour, Zoubida Benabdallah, Ibtissam Rahima Khouatria and Nassima Karima Khouatria
DOI : 10.3844/ojbsci.2012.38.43
OnLine Journal of Biological Sciences
Volume 12, Issue 2
Problem statement: Search for lactic acid bacteria that have in vitro, a significant inhibitory effect against the strains of H. pylori and to determine the inhibitory activity in vivo. Approach: The in vitro inhibitory activity of lactic acid bacteria isolated from milk against strains of H. pylori was determined by the agar diffusion method. Two groups of mice were inoculated for a week with TN2GF4. After three weeks, the infected group is treated for seven days with E. faecium (B13). H pylori was detected by a count after culture of gastric biopsy. The probiotic was determined by a count from fresh feces of mice treated. Results: Thirty strains of lactic acid bacteria were isolated and identified. E. faecium (B13) strain showed a highly significant inhibition. H. pylori was successfully detected in the gastric mucosa. E. faecium (B13) reduced the colonization in the stomach of H. pylori with a rate of 43% in a week. Conclusion: E. faecium (B13) has in vitro and in vivo an inhibitory effect against H. pylori.
© 2012 Hassina Guetarni, Ahmed Bensoltane, Djamila Aichour, Zoubida Benabdallah, Ibtissam Rahima Khouatria and Nassima Karima Khouatria. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.