Effects of Metal Ion Concentrations on Lipid and Gamma Linolenic Acid Production by Cunninghamella sp. 2A1
Farhila Muhid, Wan Nazatul Naziah Wan Nawi, Abdul Jalil Abdul Kader, Wan Mohtar Wan Yusoff and Aidil Abdul Hamid
DOI : 10.3844/ojbsci.2008.62.67
OnLine Journal of Biological Sciences
Volume 8, Issue 3
The effects of different concentration of MgSO4.7H2O, FeCl3.6H2O, ZnSO4.7H2O, CuSO4.5H2O and MnSO4.5H2O on lipid and GLA production by Cunninghamella sp. 2A1 were studied in submerged culture fermentation. Cultivation was performed in 250 ml conical flask (100ml working volume), at 30°C and 250 rpm agitation for 120h. Mg2+, Fe2+ and Zn2+ have significant effect on lipid accumulation as inclusion of each ions resulted in a 64, 43 and 33% increase in lipid content compared to cultures which was cultivated with the omission of each ions, respectively. All three metal ions also affected GLA production by Cunninghamella sp. 2A1 with Zn2+ having the most profound effect with an increase of up to 74% of GLA yield (g GLA/g lipidless biomass) achieved. As omission of any single metal ions did not affect biomass concentration achieved, we concluded that metal ions components in production media may have affected lipid and GLA production by affecting pathways involved in the biosynthesis of lipid and GLA. This study reports the critical concentration of each metal ion for optimal lipid and GLA production by Cunninghamella sp. 2A1.
© 2008 Farhila Muhid, Wan Nazatul Naziah Wan Nawi, Abdul Jalil Abdul Kader, Wan Mohtar Wan Yusoff and Aidil Abdul Hamid. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.