OnLine Journal of Biological Sciences

Construction of a Glucose Biosensor by Immobilizing Glucose Oxidase within a Poly(o-phenylenediamine) Covered Screen-printed Electrode

Miao Yuqing, Chen Jianrong and Wu Xiaohua

DOI : 10.3844/ojbsci.2006.18.22

OnLine Journal of Biological Sciences

Volume 6, Issue 1

Pages 18-22


The glucose biosensors were prepared by the electropolymerization of the non-conductive polymer, Poly(o-phenylenediamine), onto a planar screen-printed electrode. A fabrication procedure was performed to decrease the waste of expensive enzyme. The amperometric glucose response was measured by the potensiostating of the prepared glucose biosensors at a potential of 0.3 V with ferrocene as mediator. Results show that the obtained biosensors have a linear range up to 25 mM glucose, fast response time (100s) and high sensitivities (16.6 nA/mM). Also, the effects of applied potential and sweeping number of Cyclic voltammograms for electropolymerization were systematically investigated and optimal values were recorded.


© 2006 Miao Yuqing, Chen Jianrong and Wu Xiaohua. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.