In Vitro Callus Induction and Regeneration of Medicinal Plant Datura innoxia
Baan Munim Twaij, Alaa Jabbar Taha and Md. Nazmul Hasan
DOI : 10.3844/ajbbsp.2019.150.156
American Journal of Biochemistry and Biotechnology
Volume 15, Issue 3
The present study indicate the possibility of the development of callus from stem cut, leaf and root explants and regeneration of Datura plant (Datura innoxia) on Murashige and Skoog (MS) media supplemented with different concentrations of 6-Benzylaminopurine (BA) and 1-Naphthaleneacetic (NAA). Sterilized explants were inoculated and incubated in culture shade under different conditions (light and dark). Data analysis showed that maximum percentage (83%) of calli were induced from stem cut explants on MS media supplemented with NAA and BA at 0.5 and 1.0 mg/l respectively under dark condition. Maximum fresh and dry weight was found 395.63 mg and 35.64 mg respectively with supplementation of 0.5 mg/l NAA and 1 mg/l BA. Stem cut derived calli were transfer to MS regeneration medium supplementing different concentrations of plant regulators (PGRs) under different conditions. Maximum regeneration (78%) was found on MS media supplementing 1 mg/l of BA and 1 mg/l off NAA under dark condition. The attempt for callus induction from the explants (stem, leaf, root) using MS media with supplementation of different combinations of 2, 4-Dichlorophenoxyacetic acid (2, 4-D), BA and NAA, Kinetin (kin) under both light and dark conditions were carried out but no significant results were found.
© 2019 Baan Munim Twaij, Alaa Jabbar Taha and Md. Nazmul Hasan. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.