Research Article Open Access

Optimization, Characterization and Antioxidant Activities of Selenized Polysaccharides from Hypsizygus marmoreus

  • Ting Liu Changshu Institute of Technology, China
  • Xue Jiang Changshu Institute of Technology, China
  • Wei Wang Changshu Institute of Technology, China
  • Bing Xu Changshu Institute of Technology, China

American Journal of Biochemistry and Biotechnology
Volume 15 No. 3, 2019, 138-149
DOI: https://doi.org/10.3844/ajbbsp.2019.138.149

Abstract

In this study, hypsizygus marmoreus was used as raw material to extract polysaccharides by hot water extraction method. Preparation technology of Selenized Polysaccharides from Hypsizygus marmoreus (SPHM) and its antioxidant activities were investigated. Chelation rate was selected as the index, effects of initial concentration of sodium selenite (mg/mL), the mass ratio of polysaccharide to sodium selenite, chelation time and pH on the chelation rate was investigated by single factor test. Response surface method was used to optimize the preparation technology of SPHM. The preliminary structure characterization of Polysaccharides from Hypsizygus marmoreus (PHM) and SPHM was performed by infrared spectroscopy and scanning electron microscopy. The antioxidant activities of SPHM were studied by the scavenging effect of 1,1-Diphenyl-2-picrylhydrazyl radical 2,2-Diphenyl-1- (2,4,6-trinitrophenyl) Hydrazyl (DPPH) and hydroxyl radical. The results showed that the optimum preparation technology of SPHM was as follows: the initial concentration of sodium selenite was 7.95 (mg/mL), the mass ratio of polysaccharide to sodium selenite was 6.11:1 (mg/mg), chelation time was 6.89h and pH was 5.11. Under the optimum conditions, chelate rate was 10.36%. Infrared spectroscopy and scanning electron microscopy analysis showed that selenium ions successfully combined with PHM and the surface structure of PHM and SPHM was different. Compared with HPM, the antioxidant activities of SPHM enhanced.

How to Cite

Liu, T., Jiang, X., Wang, W. & Xu, B. (2019). Optimization, Characterization and Antioxidant Activities of Selenized Polysaccharides from Hypsizygus marmoreus. American Journal of Biochemistry and Biotechnology, 15(3), 138-149. https://doi.org/10.3844/ajbbsp.2019.138.149

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Keywords

  • SPHM
  • Sodium Selenite
  • Chelation
  • Antioxidant