SCANNING ELECTRON MICROSCOPE IMAGING OF AMYLOID FIBRILS
Eisuke Takai, Gai Ohashi, Ryuichi Ueki, Yoichi Yamada, Jun-Ichi Fujita and Kentaro Shiraki
DOI : 10.3844/ajbbsp.2014.31.39
American Journal of Biochemistry and Biotechnology
Volume 10, Issue 1
This study demonstrated the applicability of Scanning Electron Microscopy (SEM) for the observation of amyloid fibrils without staining. As model specimens, two types of amyloid fibrils with different shapes and chemical compositions were controllably synthesized from hen lysozyme. The apparent fibril widths in the SEM images were considerably larger than the original diameters analyzed by the conventional techniques of Transmission Electron Microscopy (TEM) and Atomic Force Microscopy (AFM). Although this broadening, which depends on the chemical nature of the fibril, is not desirable for detailed imaging, it makes SEM sensitive to fibrils several micrometers in length and as thin as 3.5 nm. Note that the sensitivity also contributed to clearly distinguishing amyloid fibrils from salt microcrystals in SEM images. These results suggest the considerable applicability of SEM for the imaging of amyloid fibrils, even in contaminated samples.
© 2014 Eisuke Takai, Gai Ohashi, Ryuichi Ueki, Yoichi Yamada, Jun-Ichi Fujita and Kentaro Shiraki. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.