Research Article Open Access

Production of Recombinant Proteins in Insect Cells

Christian Kollewe1 and Andreas Vilcinskas2
  • 1 , Germany
  • 2 Justus-Liebig University of Giessen, Germany
American Journal of Biochemistry and Biotechnology
Volume 9 No. 3, 2013, 255-271

DOI: https://doi.org/10.3844/ajbbsp.2013.255.271

Submitted On: 30 May 2013 Published On: 21 September 2013

How to Cite: Kollewe, C. & Vilcinskas, A. (2013). Production of Recombinant Proteins in Insect Cells. American Journal of Biochemistry and Biotechnology, 9(3), 255-271. https://doi.org/10.3844/ajbbsp.2013.255.271

Abstract

Among the wide range of methods and expression hosts available for the heterologous production of recombinant proteins, insect cells are ideal for the production of complex proteins requiring extensive post-translational modification. This review article provides an overview of the available insect-cell expression systems and their properties, focusing on the widely-used Baculovirus Expression Vector System (BEVS). We discuss the different strategies used to generate recombinant baculovirus vectors and show how advanced techniques for virus titer determination can accelerate the production of recombinant proteins. The stable transfection of insect cells is an alternative to BEVS which has recently been augmented with recombinase-mediated cassette exchange for site-specific gene integration. We consider the advantages and limitations of these techniques for the production of recombinant proteins in insect cells and compare them to other expression platforms.

  • 2,681 Views
  • 12,038 Downloads
  • 18 Citations

Download

Keywords

  • Recombinant Protein Production
  • Insect Expression System
  • BEVS
  • Virus Titer Assay