American Journal of Biochemistry and Biotechnology

Determination of Pesticide Residues in Banana by Using High Performance Liquid Chromatography and Gas Chromatography-Mass Spectrometry

R. Paranthaman, A. Sudha and S. Kumaravel

DOI : 10.3844/ajbbsp.2012.1.6

American Journal of Biochemistry and Biotechnology

Volume 8, Issue 1

Pages 1-6


Problem statement: The occurrence of endosulfan, carbendazim, chloropyripos in 10 banana samples in southern area of Tamilnadu, India (hill banana, karpuravalli, monthan, nendran, ney poovan, pachanadan, poovan, rasthali, red banana, robusta) was investigated. Approach: In 7 samples, Carbendazim was found at concentrations ranging from 0.002-0.11 mg kg-1. In three samples, carbendazim was not found, whereas endosulfan, chloropyripos was not detected in any sample. Results: Analysis was carried out using HPLC-UV and samples were confirmed by GC-MS. The seven samples contained carbendazim that not exceeded the FAO/WHO codex alimentarius standards for MRLs (Maximum Residue Limit) values of carbendazim pesticide on banana (whole) is 1.0 mg kg-1. Conclusion/Recommendations: Based on the HPLC results carbendazim is finding in Hill banana (0.007 mg kg-1), Monthan (0.019 mg kg-1), Nendran (0.002 mg kg-1), Pachanadan (0.007 mg kg-1), Poovan (0.016 mg kg-1), Rasthali (0.017 mg kg-1) and Robusta (0.11 mg kg-1) and carbendazim is not finding Karpuravalli, Ney poovan and Red banana. Endosulfan, Chloropyrifos and Carbendazim in Robusta Banana sample are identified by matching their retention times and characteristic ion. TIC chromatogram for a positive Robusta Banana sample.


© 2012 R. Paranthaman, A. Sudha and S. Kumaravel. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.