Antibacterial and Antioxidant Activities of Typhonium Flagelliforme (Lodd.) Blume Tuber
Syam Mohan, Ahmad Bustamam Abdul, Siddig Ibrahim Abdel Wahab, Adel Sharaf Al-Zubairi, Manal Mohamed Elhassan and Mohammad Yousif
DOI : 10.3844/ajbbsp.2008.402.407
American Journal of Biochemistry and Biotechnology
Volume 4, Issue 4
Problem Statement: Multiple drug resistance in human pathogenic micro organisms has developed due to indiscriminate use of modern antimicrobial drugs generally used in the management of infectious diseases. This increases the importance of exploiting the natural sources instead modern drugs. Approach: The antibacterial and antioxidant activity of different extracts from of Typhonium flagelliforme (L.) Blume tuber (family: Araceae) commonly called âRodent Tuberâ was assessed towards selected bacteria as well as in different antioxidant models. The antibacterial screening was carried out by disc diffusion method. Two complementary test systems, namely DPPH free radical scavenging and total phenolic compounds, were used for the antioxidant analysis. Results: Except hexane extract none of the other extracts shown anti bacterial activity against the selected strains. The hexane extract from Typhonium flagelliforme tuber had interesting activity against both the gram negative bacteria, Pseudomonas aeruginosa (11Â±1.0 mm diameter) and Salmonella choleraesuis (12Â±1.1 mm diameter). The positive control, Streptomycin had shown zone of inhibition of 20Â±1.5 mm, 20Â±1.3 mm, 23Â±1.5 mm and 23Â±1.0 mm in Methicillin Resistant Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella choleraesuis and Bacillus subtilis respectively. All the extracts were subjected to screening for their possible antioxidant activity. The DPPH assay showed that the inhibitory activity of ethyl acetate (77.6Â±0.9%) and dichloromethane (70.5Â±1.7%) extracts were having comparatively admirable inhibition capacity when compared to the positive control BHT (95.3Â±1.3%). Total phenolic content of all extracts was also evaluated, and dichloromethane extracts (5.21Â±0.82 GAE mg/g extract) was superior to all other extracts, followed by hexane and ethyl acetate. Conclusion: Considering all the results collectively T. flagelliforme appears to be a promising plant demonstrating antibacterial and antioxidant activity that requires further investigation.
© 2008 Syam Mohan, Ahmad Bustamam Abdul, Siddig Ibrahim Abdel Wahab, Adel Sharaf Al-Zubairi, Manal Mohamed Elhassan and Mohammad Yousif. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.