American Journal of Biochemistry and Biotechnology

Effect of UV-C on Drought Tolerance of Alfalfa (Medicago sativa) Callus

Ali A. Ehsanpour and Roya Razavizadeh

DOI : 10.3844/ajbbsp.2005.107.110

American Journal of Biochemistry and Biotechnology

Volume 1, Issue 2

Pages 107-110


Water stress and unfavorable climate decrease the growth and development of plant globally. Medicago is a valuable plant as a source of food for animal, foliage and for medicine. Since, Iran is located in a dry area; consequently, the study of plant responses to water stress is important. In this research UV-C radiation was used to induce physiological and genetic changes in alfalfa callus. In vitro grown calluses of M. sativa were exposed to UV-C for 0, 15, 30 and 60 min and then, were transferred to MS medium containing 0, 2, 5, 10, 20, 30% PEG. All explants were kept in the dark in the culture room. Calluses tolerate to osmotic stress were selected according to fresh weight. Results showed that radiation of UV-C for 60 min increased the osmotic tolerance and decreased the effect of drought stress.


© 2005 Ali A. Ehsanpour and Roya Razavizadeh. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.