Research Article Open Access

Diversity of the Rhizosphere Soil Culture-Dependent Fungi of Mature Tobacco

Xiaolin Li1, Xinzhu Li1, Kewei Zhao1, Ran Wang1 and Xiaoping Zhang1
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Current Research in Microbiology
Volume 2 No. 1, 2011, 9-14

DOI: https://doi.org/10.3844/ajmsp.2011.9.14

Published On: 26 October 2011

How to Cite: Li, X., Li, X., Zhao, K., Wang, R. & Zhang, X. (2011). Diversity of the Rhizosphere Soil Culture-Dependent Fungi of Mature Tobacco. Current Research in Microbiology, 2(1), 9-14. https://doi.org/10.3844/ajmsp.2011.9.14

Abstract

Problem statement: The maturation of tobacco is a very important period of tobacco production. Until recently, there are no many reports on tobacco rhizospheric culturable fungi, especially at the mature stage. Approach: Five rhizosphere soils of tobacco and five non-rhizosphere soils of tobacco were collected in Yanbian county, Panzhihua. Sixty-one fungal strains isolated from these soil samples were analyzed by 18S rDNA PCR-RFLP. And 14 representative strains of them were chosen for 18S rDNA sequencing. Results: The results indicated that most of the quantity of fungi of most rhizosphere soils was bigger than rhizosphere’s. All strains can be clustered together at similar of 67% in the analyzing of 18S rDNA PCR-RFLP. The strains came from one sample were not always clustered together always, whereas the strains which came from rhizosphere soils or non-rhizosphere soils often clustered together. Conclusion: The results of 18S rDNA sequencing showed that dominant fungal species of non-rhizosphere soils were more abundant than rhizosphere. The culture-dependent fungal quantity, community structure and diversity of rhizosphere soil of mature tobacco were affected by the worsening environment of later stage of tobacco.

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Keywords

  • Mature tobacco
  • culture-dependent fungi
  • non-rhizosphere soils
  • rhizosphere soils
  • culturable fungi
  • 18s rDNA sequencing
  • worsening environment