TY  - JOUR
AU  - Li, Xiaolin 
AU  - Li, Xinzhu 
AU  - Zhao, Kewei 
AU  - Wang, Ran 
AU  - Zhang, Xiaoping 
PY  - 2011
TI  - Diversity of the Rhizosphere Soil Culture-Dependent Fungi of Mature Tobacco
JF  - Current Research in Microbiology
VL  - 2
IS  - 1
DO  - 10.3844/ajmsp.2011.9.14
UR  - https://thescipub.com/abstract/ajmsp.2011.9.14
AB  - Problem statement: The maturation of tobacco is a very important period of tobacco production. Until recently, there are no many reports on tobacco rhizospheric culturable fungi, especially at the mature stage. Approach: Five rhizosphere soils of tobacco and five non-rhizosphere soils of tobacco were collected in Yanbian county, Panzhihua. Sixty-one fungal strains isolated from these soil samples were analyzed by 18S rDNA PCR-RFLP. And 14 representative strains of them were chosen for 18S rDNA sequencing. Results: The results indicated that most of the quantity of fungi of most rhizosphere soils was bigger than rhizosphere’s. All strains can be clustered together at similar of 67% in the analyzing of 18S rDNA PCR-RFLP. The strains came from one sample were not always clustered together always, whereas the strains which came from rhizosphere soils or non-rhizosphere soils often clustered together. Conclusion: The results of 18S rDNA sequencing showed that dominant fungal species of non-rhizosphere soils were more abundant than rhizosphere. The culture-dependent fungal quantity, community structure and diversity of rhizosphere soil of mature tobacco were affected by the worsening environment of later stage of tobacco.