@article {10.3844/ajmsp.2011.9.14,
article_type = {journal},
title = {Diversity of the Rhizosphere Soil Culture-Dependent Fungi of Mature Tobacco},
author = {Li, Xiaolin and Li, Xinzhu and Zhao, Kewei and Wang, Ran and Zhang, Xiaoping},
volume = {2},
year = {2011},
month = {Oct},
pages = {9-14},
doi = {10.3844/ajmsp.2011.9.14},
url = {https://thescipub.com/abstract/ajmsp.2011.9.14},
abstract = {Problem statement: The maturation of tobacco is a very important period of tobacco production. Until recently, there are no many reports on tobacco rhizospheric culturable fungi, especially at the mature stage. Approach: Five rhizosphere soils of tobacco and five non-rhizosphere soils of tobacco were collected in Yanbian county, Panzhihua. Sixty-one fungal strains isolated from these soil samples were analyzed by 18S rDNA PCR-RFLP. And 14 representative strains of them were chosen for 18S rDNA sequencing. Results: The results indicated that most of the quantity of fungi of most rhizosphere soils was bigger than rhizosphere’s. All strains can be clustered together at similar of 67% in the analyzing of 18S rDNA PCR-RFLP. The strains came from one sample were not always clustered together always, whereas the strains which came from rhizosphere soils or non-rhizosphere soils often clustered together. Conclusion: The results of 18S rDNA sequencing showed that dominant fungal species of non-rhizosphere soils were more abundant than rhizosphere. The culture-dependent fungal quantity, community structure and diversity of rhizosphere soil of mature tobacco were affected by the worsening environment of later stage of tobacco.},
journal = {Current Research in Microbiology},
publisher = {Science Publications}
}