Diversity of the Rhizosphere Soil Culture-Dependent Fungi of Mature Tobacco
Xiaolin Li, Xinzhu Li, Kewei Zhao, Ran Wang and Xiaoping Zhang
DOI : 10.3844/ajmsp.2011.9.14
Current Research in Microbiology
Volume 2, Issue 1
Problem statement: The maturation of tobacco is a very important period of tobacco production. Until recently, there are no many reports on tobacco rhizospheric culturable fungi, especially at the mature stage. Approach: Five rhizosphere soils of tobacco and five non-rhizosphere soils of tobacco were collected in Yanbian county, Panzhihua. Sixty-one fungal strains isolated from these soil samples were analyzed by 18S rDNA PCR-RFLP. And 14 representative strains of them were chosen for 18S rDNA sequencing. Results: The results indicated that most of the quantity of fungi of most rhizosphere soils was bigger than rhizosphere’s. All strains can be clustered together at similar of 67% in the analyzing of 18S rDNA PCR-RFLP. The strains came from one sample were not always clustered together always, whereas the strains which came from rhizosphere soils or non-rhizosphere soils often clustered together. Conclusion: The results of 18S rDNA sequencing showed that dominant fungal species of non-rhizosphere soils were more abundant than rhizosphere. The culture-dependent fungal quantity, community structure and diversity of rhizosphere soil of mature tobacco were affected by the worsening environment of later stage of tobacco.
© 2011 Xiaolin Li, Xinzhu Li, Kewei Zhao, Ran Wang and Xiaoping Zhang. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.