Development of Growth Medium for Centella Asiatica Cell Culture Via Response Surface Methodology
Rozita Omar, M. A. Abdullah, M. A. Hasan and M. Marziah
DOI : 10.3844/ajassp.2004.215.219
American Journal of Applied Sciences
Volume 1, Issue 3
The effects of sucrose, Indole-3-Acetic Acid (IAA) and 6-BenzylAminoPurine (BAP) concentrations on cell growth of Centella asiatica cell suspension culture were studied. The concentrations were designed using Central-composite experimental design and regression analysis was carried out to obtain response surface model describing cell growth for prediction of optimum conditions. Only sucrose as a single factor was positively significant for cell growth. Increasing sucrose concentration from 3.32 to 6.68% (w/v) resulted in an increase in dry cell weight from 16-27 g L-1. IAA and BAP as single factors and other possible interaction effect were insignificant. The optimum values predicted to be 6.68% (w/v) sucrose, 0.84 mg L-1 IAA and 1.17 mg L-1 BAP yielding 27.4 g L-1 dry cell weight with 81.4% regression equation fitness of the experimental data.
© 2004 Rozita Omar, M. A. Abdullah, M. A. Hasan and M. Marziah. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.