@article {10.3844/ajassp.2004.215.219,
article_type = {journal},
title = {Development of Growth Medium for Centella Asiatica Cell Culture Via Response Surface Methodology},
author = {Omar, Rozita and Abdullah, M. A. and Hasan, M. A. and Marziah, M.},
volume = {1},
year = {2004},
month = {Sep},
pages = {215-219},
doi = {10.3844/ajassp.2004.215.219},
url = {https://thescipub.com/abstract/ajassp.2004.215.219},
abstract = {The effects of sucrose, Indole-3-Acetic Acid (IAA) and 6-BenzylAminoPurine (BAP) concentrations on cell growth of Centella asiatica cell suspension culture were studied. The concentrations were designed using Central-composite experimental design and regression analysis was carried out to obtain response surface model describing cell growth for prediction of optimum conditions. Only sucrose as a single factor was positively significant for cell growth. Increasing sucrose concentration from 3.32 to 6.68% (w/v) resulted in an increase in dry cell weight from 16-27 g L-1. IAA and BAP as single factors and other possible interaction effect were insignificant. The optimum values predicted to be 6.68% (w/v) sucrose, 0.84 mg L-1 IAA and 1.17 mg L-1 BAP yielding 27.4 g L-1 dry cell weight with 81.4% regression equation fitness of the experimental data.},
journal = {American Journal of Applied Sciences},
publisher = {Science Publications}
}