TY  - JOUR
AU  - Omar, Rozita 
AU  - Abdullah, M. A. 
AU  - Hasan, M. A. 
AU  - Marziah, M. 
PY  - 2004
TI  - Development of Growth Medium for Centella Asiatica Cell Culture Via Response Surface Methodology
JF  - American Journal of Applied Sciences
VL  - 1
IS  - 3
DO  - 10.3844/ajassp.2004.215.219
UR  - https://thescipub.com/abstract/ajassp.2004.215.219
AB  - The effects of sucrose, Indole-3-Acetic Acid (IAA) and 6-BenzylAminoPurine (BAP) concentrations on cell growth of Centella asiatica cell suspension culture were studied. The concentrations were designed using Central-composite experimental design and regression analysis was carried out to obtain response surface model describing cell growth for prediction of optimum conditions. Only sucrose as a single factor was positively significant for cell growth. Increasing sucrose concentration from 3.32 to 6.68% (w/v) resulted in an increase in dry cell weight from 16-27 g L-1. IAA and BAP as single factors and other possible interaction effect were insignificant. The optimum values predicted to be 6.68% (w/v) sucrose, 0.84 mg L-1 IAA and 1.17 mg L-1 BAP yielding 27.4 g L-1 dry cell weight with 81.4% regression equation fitness of the experimental data.