Factors Affecting the Geometry of Silver Nanoparticles Synthesis in Chrysosporium Tropicum and Fusarium Oxysporum

Abstract

Problem statement: Biosynthesis of nanoparticles using fungal cells is a novel approach to develop biotechnological possess such as bioleaching and bioremediation. In the present study, an effort was made to investigate the effect of physio-chemical parameters on the silver nanoparticle formation with the fungus Chrysosporium tropicum Carmichael and Fusarium oxysporum Schltdl. Approach: The possibilities to manipulating the geometry of silver nanoparticles by altering the key growth parameters such as pH, temperature, concentrations and time have been explored. The effect of AgNO₃ with the cell free extract of fungi and time, temperature, pH with the formation of silver has also been investigated. The presence of nanosilver has been carried out with the Micro-scan reader and has been confirmed by X-Rays Diffractometer (XRD). The micrographs of the silver nanoparticles have been evaluated through the Transmission Electron Microscope (TEM) and confirmed by Scanning Electron Microscope (SEM). The effect of concentrations with response to time, temperature and pH has studied with the help of Micro-scan reader and their microstructure analyzed by TEM and SEM. Results: It was observed that fungus C. tropicum and F. oxysporum Schltdl significantly activate the extra-cellular production of silver nanoparticles. The different sized and spherical shaped nanoparticles have been formed in different strains. With the increase in concentration, the absorbance increased with response to time (24-120h) and temperature. Significantly, the pH was found decreasing with the increase of absorbance. Conclusion: We presume that these changes initiate new geometry of nanosilver in the cell free solutions. These different shaped, sized and geometry of nanoparticles can be used in the field of medicine for drug formation and diseases diagnosis.