Research Article Open Access

Comparison of IncL/M Plasmids Using the Neighbor-Joining Method on Basis repA and excA Genes

Touati Abdelaziz1 and Mairi Assia1
  • 1 Université de Bejaia, Algeria

Abstract

The aim of this study is to make comparison of IncL/M groups on basis of two genes candidates’ repA and excA genes. The sequences of 27 plasmids were compared using the neighbor-joining method. This method was used to construct a phylogenetic tree for the nucleotide sequences of two genes (repA and excA), using the program MEGA X software. The evolutionary distances were computed using the maximum composite likelihood method. The neighbor-joining method analysis showed different results based on the gene used for comparison. The repA gene was more accurate than excA gene to distinguish between different incL/M plasmids. This study suggested that IncL/M plasmids harboring different antibiotic resistance genes have evolved differently.

Current Research in Bioinformatics
Volume 8 No. 1, 2019, 14-17

DOI: https://doi.org/10.3844/ajbsp.2019.14.17

Submitted On: 25 December 2019 Published On: 27 February 2020

How to Cite: Abdelaziz, T. & Assia, M. (2019). Comparison of IncL/M Plasmids Using the Neighbor-Joining Method on Basis repA and excA Genes. Current Research in Bioinformatics, 8(1), 14-17. https://doi.org/10.3844/ajbsp.2019.14.17

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Keywords

  • Plasmids
  • IncL/M Type
  • Phylogenetic Analysis
  • repA Gene
  • excA Gene